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Timeline 2007
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A Timeline of Biotechnology 540
August 28, 2007

 

Class Questions - Discuss with a partner

 

(I) Nature, January 2003: The Birth of Biotechnology
* Why were Cohen and Boyer at the same meeting in Hawaii in 1972?

* Cohen studied:

* Boyer studied:

* Why do bacteria make restriction enzymes?


* Of what use to bacteria are plasmids?


* What was the idea Cohen and Boyer discussed (in the Deli) to get their two research programs together?

(II) From Morrow et al (Cohen and Boyers original paper, May 1974) 1. What is pSC101?

2. What was the source of the DNA cloned into pSC 101?

3. Fig 2 - What is the 5.8 kb fragment?

4. Fig 2 - What are CD42, CD30, CD35, CD4, and CD18?

5. What is a minicell and what does it have to do with this procedure?

 

6. In retrospect, why do you think they chose to clone rDNA into pSC101 rather than another gene?


7. Read the last sentence of this paper, and say "like wow..."

 

 

 

(III) Potential Biohazards of rDNA molecules July 1974

 

1. Who authored this article?

 

2. Why was the use of E. coli of particular concern these scientists?

 

 

 

3. What were the 4 recommendations of the authors?

First:

Second:

Third:
Fourth:

Last sentence?

 

 

 

(IV) Summary Staetment from Asilomar: Paul Berg, Maxine Singer June 1975

 

1. What were the 2 major concerns of Asilomar participants (second paragraph)

 

2. What two types of 'biological barriers' do the authors recommend for containment (top of pg 1982)?

 

3. Please be sure to glance over (NOT commit to memory) the

 

 

Objectives specific to this lecture

1.Identify Nobel-winning scientists and their research stated above. Why? because every self-respecting Biology major SHOULD!
2.Explain how the Cohen-Boyer partnership resulted in 'the birth of biotech' (aka Cloned beef to cloning), the role of Robert Swanson developing the biotechnology company Genentech

3.Explain the major results of the Cohen-Boyer 1974 PNAS paper. We will go over this paper in class Tuesday evening
4. What were the major recommendations of the 1974 paper "Potential Biohadards of Recombinant DNA molecules? What was the outcome of this manuscript?

5. What were the major conclusions from Asilomar conference and what are their significance in the use of recombinant DNA technology? Be able to discuss the the initial concerns and recommendations that surround this important event.



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Copyright Kathleen A. Marrs 1998-2007